The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. As. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). i3x. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. 04, 0. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Find regional support. Eliminate time-dependent substrate addition steps. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Actual shake speed is based on the microplate format. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. detection cartridge LUM96: 59. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. 312 gain calibration failed. The SpectraMax i3x reader provides the With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. 발광 (Luminescence) 리더기. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Examples: fiPress carefully to engage,fl fiDo not press down. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Other services inlcude but are not limited to anti-drug activity (ADA), PK/PD assays as well as antibody screenings. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Published Dec 16, 2020 | Updated Oct 10, 2023. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Prepare your i3/i3x instrument for shipment. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes This assay workflow is easily performed using the SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge, which is fully DL Ready validated. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 660-665 Eskdale Road. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. com Check our website for a current listing of worldwide distributors. Note: Additional readers with similar performance are listed. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. Multi-Mode Reader. Bioz Stars score: 98/100, based on 1 PubMed citations. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 吸収度マイクロプレートリーダー SpectraMax® Mini. The calculated LOQ values were 0. 200-800 nm using Spectra Max i3X microplate reader. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. The treatments were. Cell counters for accurate and. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. SpectraMax i3x and Paradigm readers require the FP-FLUO. Synthesis of NORP Synthesis of compound 1. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 2 in. Note: Additional readers with similar performance are listed. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthSpectraMaxABSInstrumentInstallationGuide 4 5065391A ComputerIntegration EachMolecularDevicesmicroplatereaderisshippedwithalicensekeyfortheSoftMax®ProSpectraMax iD5 reader. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Nuclear Receptor. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Each heating element is independently. The MPO assay was conducted according to the. 흡광 (Absorbance) 리더기. Features. Molecular Devicesの全商品を見る. . 0. Fluorescence Microplate Reader Comparison Chart. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Download PDF. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. The plate was shaken and incubated in the dark for 10 minutes. Introduction. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. 1. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. D. 58% for the detection module and 2. 0001 0. The standard solutions (0. The chamber. 2 (Molecular Devices, San Jose, CA). Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. This is not due to a temperature-dependent alteration in cellular autofluorescence,. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Microplate Stacker. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. ISM Equipment Classification (Group 1, Class A) This equipment is. We would like to show you a description here but the site won’t allow us. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. , 2014; Xiong et al. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. In addition, Image J was used to quantify the. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. XPS spectra were obtained from PHI Versaprobe II. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. 001 0. The treatments were. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Microplate optimization should be performed the first time the assay is run, and subsequently for . Find regional support. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Description 1. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. Find our technical support information along with contact details. 1. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. Thetypeof. All test measurements and calculations are handlediD5. Figure 4. 00800 665 32860. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). 316 grating motor driver faulty. Modularity does provide some measure of forward vision into upcoming or future experimental needs. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. Fluorescent Polarization: Solid black plates, as above. This way you’ll save time on finding the necessary info. Image analysis provides accurate data. i3. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. Nucleic Acid and Protein Quantitation. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. Multimode Reader Comparison Chart. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. *On the SpectraMax i3x reader only. StainFree Cell Detection Algorithm. 304 signal level saturation. Patented user-exchangeable cartridge design expands the system's detection capability making it. 1. 0. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. ) deep, preferably with access on all sides. 4 mg mL −1; 1. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Explore a wealth of applications with the future-ready SpectraMax i3x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. ZERO BIAS - scores, article reviews, protocol conditions and more. Figure 3. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. The size and fluorescence intensity were tested for 48 h. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Thetypeof luciferase(Renilla. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Myeloperoxidase (MPO) Assay. Category 2 and 3 microplate readers use gradient heating as well. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. The SoftMax Pro Software provides integrated instrument control. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Find regional support. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Get a front row view of your cells. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. When the user applies a set temperature of 37°C, the top is heated to 37. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. Published Dec 16, 2020 | Updated Dec 16, 2020. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. 2 only) SpectraMax M-series Microplate readers. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. Find regional support. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Italic and boldface type are used for emphasis. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. 314 low lamp level warning. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Reporter Assay. Examples: fiPress carefully to engage,fl fiDo not press down. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. SpectraMax i3/i3x readers must be equipped with a suitableWhen we measured the FRET ratio using the Spectramax i3x plate reader, we found that FRET ratios were higher in DMSO-treated cells that had not been incubated at room temperature to enhance fluorescence than in room temperature-incubated cells (Fig. Table 1. The corrected absorbance is within 5% of the. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. eu@moldev. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 4 / 5. 99. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. ) wide and 65 cm (25. Description 1. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. However, no piece of equipment is immune to breaking down. 価格、見積もり、お近くの販売店を. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. 5 µL anti-cAMP-Cryptate. Eliminate time-dependent substrate addition steps. Find regional support. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). 5. It allows you to explore cellular pathways and protein activation and expression in one system. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology*On the SpectraMax i3x reader only. Louis, MO) and used as received without further purification. ) deep, preferably with access on all sides. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. SpectraMax ® iD3 and iD5 Multi-Mode . 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Unlock your i3/i3x instrument after shipment. no. Plate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 313 reference gain check fail. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 価格、見積もり、お近くの販売店を. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. 제품. , 1997). SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. SpectraMax i3x Multi-Mode Detection Platform. Promega. TUNE Tunable Wavelength Detection Cartridge. Description 1. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SoftMax Pro. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. 4. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Features. 0´ 104 1. 2 in. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. It allows you to explore cellular pathways and protein activation and expression in one system. 1. 1step assay with SpectraMax microplate readers. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The corrected absorbance is within 5% of the value. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Microplate readers, multi-mode, SpectraMax® i3x. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. 6 in. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. HTRF-compatible SpectraMax Multi-Mode Readers. Winnersh Triangle. 2. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax ® i3x. Data Analysis. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. 蛍光 発光 ELISA. Read height should be optimized for . Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Patented user-exchangeable cartridge design expands the system's detection capability making it. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Time-. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. ATP (“flash” luminescence using . SpectraMax Microplate Readers. 8 Antibodies and. The calculated LOQ values were 0. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. • Removing the transport locks from the PMT shutters and drawers. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Molecular Devices are known for both the reliability and the wide uses of their products. 9 x 10-15. 2. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Figure 1. Overview of SpectraMax MiniMax 300 Imaging Cytometer. See Unpacking the SpectraMax Paradigm Instrument on page 1. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. 99 to $1999. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Prepare your i3/i3x instrument for shipment. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. 2. Molecular Devicesの全商品を見る. Italic and boldface type are used for emphasis. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 08, 0. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Supplier Page. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. HTRF IP standard curve. 3) At the bottom of the strip, click on "Show. The turbidity. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Hands-on fun and learning that empowers exploring, inventing and sharing. How to properly unlock iD series instrument. 6% for the on-board optics (monochromators). The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Unlock your i3/i3x instrument after shipment. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers.